No significant correlations were detected between the physicochemical and microbiological parameters with the presence of H. capsulatum was previously detected, suggesting the persistence of the fungus. Seven of the positive samples represented locations where H. A total of 25 (10.5%) samples were positive and validated by sequencing. The Hc100 nested PCR had a detection limit of 0.1 pg/µL and a specificity of 100%. A total of 239 samples were collected: 201 (84.1%) corresponded to organic fertilizers, 30 (12.5%) to bird excrement, and 8 (3.4%) to cave soils. capsulatum presence in organic fertilizers by nested polymerase chain reaction (PCR). The aim of this study was to demonstrate H. The Instituto Colombiano Agropecuario resolution 150 of 2003 contains the parameters set by the Colombian Technical Standard (NTC 5167) on the physicochemical and microbiological features of fertilizers, but it does not regulate the search for H. Cases and outbreaks due to the presence of the fungus in these components have been reported. The replacement of organic matter in agroecosystems is necessary in the tropics, and the use of organic fertilizers has increased. Taylor, Fungal Immunology Laboratory, Universidad Nacional Autonoma de Mexico, Coyoacan, Distrito Federal, Mexico, E-mail: capsulatum is the causative agent of histoplasmosis and this fungus inhabits soils rich in phosphorus and nitrogen that are enriched with bird and bat manure. Torres, School of Medicine, Universidad CES, Medellin, Antioquia, Colombia, E-mail: Catalina de Bedout, Medical and Experimental Mycology Group, Corporación para Investigaciones Biológicas (CIB), Medellin, Colombia, E-mail: Carlos A Peláez and Jose M Acevedo, Molecular Studies Interdisciplinary Group, Chemistry Institute, Natural and Exact Sciences, Universidad de Antioquia, Medellín, Colombia, E-mails: and María L. McEwen, Myrtha Arango and María del Pilar Jiménez-A, Department of Microbiology and parasitology, School of Medicine, Universidad de Antioquia, Medellin, Antioquia, Colombia, E-mails: and and Isaura P.
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